Studies on fluorinated pyrimidines. V. Effects on nucleic acid metabolism in vitro.
نویسندگان
چکیده
To gain further information on the mechanisms by which the fluorinated pyrimidines and their nucleosides inhibit various processes of nucleic acid biosynthesis, studies similar to those re ported in the preceding paper (6) were carried out in vitro in glycolyzing suspensions of the Ehrlich ascites cells system used by LePage for studies of purine biosynthesis (20). In this work the following labeled precursors were used: formate-C14, uracil-2-C14, orotic acid-6-C14, thymidine-6-H3, phosphate-P32, 5-fluorouracil-2-C14, and 5-fluoroorotic-2-C14 acid.
منابع مشابه
Studies on Fluorinated Pyrimidines
We have previously reported on the tumor inhibitory properties of 5-fluorouracil and 5-fluoroorotic acid (3, 4), prepared by Duschinsky et al. (5), and on the initial clinical trials of 5fluorouracil with patients with far advanced solid tumors (6). The metabolism of 5-fluorouracil and 5-fluororotic acid has been studied, data on excretion and tissue distribution in mice and man have been repor...
متن کاملStudies on fluorinated pyrimidines. VIII. Further biochemical and metabolic investigations.
We have previously reported on the tumor inhibitory properties of 5-fluorouracil and 5-fluoroorotic acid (3, 4), prepared by Duschinsky et al. (5), and on the initial clinical trials of 5fluorouracil with patients with far advanced solid tumors (6). The metabolism of 5-fluorouracil and 5-fluororotic acid has been studied, data on excretion and tissue distribution in mice and man have been repor...
متن کاملStudies on fluorinated pyrimidines. III. The metabolism of 5-fluorouracil-2-C14 and 5-fluoroorotic-2-C14 acid in vivo.
As one of the initial steps towards the determi nation of the metabolism and mechanism of action of fluorinated pyrimidines, 5-fluorouracil and 5fluoroorotic acid were labeled with radiocarbon in the 2-carbon atom. We have previously reported briefly that fluorouracil is incorporated, as such, into the combined nucleic acids of mouse liver, spleen, and Ehrlich ascites cells (9). The present rep...
متن کاملStudies on fluorinated pyrimidines. IV. Effects on nucleic acid metabolism in vivo.
In conjunction with the studies on the metabo lism and incorporation of labeled 5-fluorouracil and 5-fluoroorotic acid into RNA (2), investigations have been carried out to determine the effects of various fluorinated pyrimidine derivatives as in hibitors of nucleic acid biosynthesis. Because the fluorine atom in the pyrimidine molecule is at tached to the same carbon that bears the methyl grou...
متن کاملExperimental and Clinical Use of Fluorinated Pyrimidines in Cancer Chemotherapy.
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ورودعنوان ژورنال:
- Cancer research
دوره 18 3 شماره
صفحات -
تاریخ انتشار 1958